human erα bc cell line mcf7  (ATCC)

Journal: Breast Cancer: Targets and Therapy

Article Title: Profiling of gene expression regulated by 17β-estradiol and tamoxifen in estrogen receptor-positive and estrogen receptor-negative human breast cancer cell lines

doi: 10.2147/bctt.s146247

Figure Lengend Snippet: Figure 1 Cluster analysis of the time course of E2-regulated gene expression in (A) MCF7, (B) T47D, (C) BT474, and (D) SKBR3 cells. Gene cluster analysis was performed for 84 genes after E2 exposure at 24 and 48 h. The threshold cycle (Ct) values were used to calculate the FCs in gene expression (treated cells vs controls – untreated cells) using the PCR array data analysis software (SABiosciences™), which was based on the 2−ΔΔCt method. Significant differentially expressed genes (FC=±2; P<0.05) were selected to graphically evaluate the activation or inhibition of gene expression in each treated cell line. Induced genes are shown in red, suppressed genes in green, and unregulated genes in black. Abbreviations: E2, 17β-estradiol; FC, fold change.

Article Snippet: Human BC cell lines MCF7 (ERα+/HER2−), T47D (ERα+/ HER2−), BT474 (ERα+/HER2+), and SKBR3 (ERα−/ HER2+) were obtained from the American Type Culture Collection (Manassas, VA, USA).

Techniques: Gene Expression, Software, Activation Assay, Inhibition

Journal: Breast Cancer: Targets and Therapy

Article Title: Profiling of gene expression regulated by 17β-estradiol and tamoxifen in estrogen receptor-positive and estrogen receptor-negative human breast cancer cell lines

doi: 10.2147/bctt.s146247

Figure Lengend Snippet: Figure 2 Cluster analysis of the time course of TAM-regulated gene expression in (A) MCF7, (B) T47D, (C) BT474, and (D) SKBR3 cells. Gene cluster analysis was performed for 84 genes after TAM exposure at 24 and 48 h. The threshold cycle (Ct) values were used to calculate the FCs in gene expression (treated cells vs controls – untreated cells) using the PCR array data analysis software (SABiosciences™), which was based on the 2−ΔΔCt method. Significant differentially expressed genes (FC=±2; P<0.05) were selected to graphically evaluate the activation or inhibition of gene expression in each treated cell line. Induced genes are shown in red, suppressed genes in green, and unregulated genes in black. Abbreviations: TAM, tamoxifen; FC, fold change.

Article Snippet: Human BC cell lines MCF7 (ERα+/HER2−), T47D (ERα+/ HER2−), BT474 (ERα+/HER2+), and SKBR3 (ERα−/ HER2+) were obtained from the American Type Culture Collection (Manassas, VA, USA).

Techniques: Gene Expression, Software, Activation Assay, Inhibition

Journal: Breast Cancer: Targets and Therapy

Article Title: Profiling of gene expression regulated by 17β-estradiol and tamoxifen in estrogen receptor-positive and estrogen receptor-negative human breast cancer cell lines

doi: 10.2147/bctt.s146247

Figure Lengend Snippet: Figure 3 PCA of MCF7, T47D, BT474, and SKBR3 cells after E2 and TAM treatments. PCA was performed for all evaluated genes (84 genes) compared with time 0. Thus grouped, after 24 and 48 h of E2 treatment, MCF7 and BT474 cells showed most diverse conditions compared with the control (time 0) and also between them, differing in the direction of the PC1 and PC2, respectively, while after TAM treatment, the cells had a lesser difference compared with the control (time 0). The largest response after TAM treatment for 24 and 48 h was found in MCF7 cells. Each cell line is represented by a different color, and each treatment performed is represented by a different geometric figure. Abbreviations: PCA, principal component analysis; E2, 17β-estradiol; TAM, tamoxifen; PC, principal component.

Article Snippet: Human BC cell lines MCF7 (ERα+/HER2−), T47D (ERα+/ HER2−), BT474 (ERα+/HER2+), and SKBR3 (ERα−/ HER2+) were obtained from the American Type Culture Collection (Manassas, VA, USA).

Techniques: Control